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glut5  (Boster Bio)


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    Structured Review

    Boster Bio glut5
    Glut5, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+slc2a5+antibodies/Anti-Glucose+Transporter+5+GLUT5%2FSLC2A5+Antibody+Picoband/pm37839228-96-97-102
    Average 92 stars, based on 1 article reviews
    glut5 - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Gene Expression:

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration.
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Staining:

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration.
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Proliferation Assay:

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration.
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Cell Culture:

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration.
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    MTS Assay:

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration.
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    CRISPR:

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration.
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    In Situ:

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration.
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Clone Assay:

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration.
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Disruption:

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration.
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Western Blot:

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration.
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Control:

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration.
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Expressing:

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration.
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Wound Healing Assay:

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration.
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Functional Assay:

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration.
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Mutagenesis:

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration.
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Transfection:

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration.
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Electron Microscopy:

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration.
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    In Vitro:

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration.
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    In Vivo:

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration.
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Membrane:

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration.
    Article Snippet: Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.Anti-β-tubulin antibodies were from Thermo Fisher (Cat# MA5-16308) and used at a dilution of 1:2000; anti-GAPDH antibodies (Cat# Ab8245) and anti-mitofusin-1 antibodies (Cat# ab129154) were purchased from Abcam and used at the dilution of 1:1000.. Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.. Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.Anti-Flag tag antibodies were from Thermo Fisher (Cat# 740001) and used at 1:100 dilution.



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    KLC3 Activates ERK1/2 and p38 Signaling in Gastric Cancer Cells. A Differential mRNA Expression Volcano Plot: A volcano plot illustrates the changes in protein expression levels upon KLC3 modulation in gastric cancer cells. Downregulated proteins are depicted as blue circles on the left, and upregulated proteins are shown as red circles on the right. The x-axis represents the fold change in expression, while the y-axis indicates the statistical significance (P value). B qRT-PCR Validation of Key Differentially Expressed Genes: The top five genes exhibiting the most significant reduction in expression among all differentially expressed genes were validated using qRT-PCR. C Differential Protein Expression Heatmap: A heatmap visualizes the differential expression of proteins, with upregulated proteins shown in red and downregulated proteins in blue. D Top Five Enriched KEGG Pathways: A bar chart identifies the five most significantly enriched KEGG pathways based on differential gene expression. The y-axis lists the KEGG pathway names, and the x-axis indicates the corresponding P values. E KEGG Classification Map: A classification map of differentially expressed genes according to their involvement in KEGG metabolic pathways. The vertical axis represents the KEGG pathway names, and the horizontal axis shows the number of genes annotated to each pathway and their proportion to the total number of annotated genes. F KLC3-SLC2A5 Protein Interaction Analysis: Western blot analysis confirms the interaction between KLC3 and SLC2A5 proteins in AGS gastric cancer cells. G p38 MAPK Signaling Activation: Western blot analysis reveals the levels of phosphorylated p38 (p-p38) and total p38 proteins in HGC-27, MKN-45, and AGS cells following KLC3 knockdown. ** P < 0.05, ** P < 0.01, *** P < 0.001 indicate statistical significance. H ERK1/2 Signaling Activation: Western blot analysis shows the levels of phosphorylated ERK1/2 (p-ERK1/2) and total ERK1/2 proteins in HGC-27, MKN-45, and AGS cells after KLC3 knockdown. ** P < 0.05, ** P < 0.01, *** P < 0.001 denote statistical significance

    Journal: BMC Cancer

    Article Title: KLC3 drives gastric cancer progression by stabilizing SLC2A5 to activate MAPK signaling and promote epithelial-mesenchymal transition

    doi: 10.1186/s12885-025-15084-x

    Figure Lengend Snippet: KLC3 Activates ERK1/2 and p38 Signaling in Gastric Cancer Cells. A Differential mRNA Expression Volcano Plot: A volcano plot illustrates the changes in protein expression levels upon KLC3 modulation in gastric cancer cells. Downregulated proteins are depicted as blue circles on the left, and upregulated proteins are shown as red circles on the right. The x-axis represents the fold change in expression, while the y-axis indicates the statistical significance (P value). B qRT-PCR Validation of Key Differentially Expressed Genes: The top five genes exhibiting the most significant reduction in expression among all differentially expressed genes were validated using qRT-PCR. C Differential Protein Expression Heatmap: A heatmap visualizes the differential expression of proteins, with upregulated proteins shown in red and downregulated proteins in blue. D Top Five Enriched KEGG Pathways: A bar chart identifies the five most significantly enriched KEGG pathways based on differential gene expression. The y-axis lists the KEGG pathway names, and the x-axis indicates the corresponding P values. E KEGG Classification Map: A classification map of differentially expressed genes according to their involvement in KEGG metabolic pathways. The vertical axis represents the KEGG pathway names, and the horizontal axis shows the number of genes annotated to each pathway and their proportion to the total number of annotated genes. F KLC3-SLC2A5 Protein Interaction Analysis: Western blot analysis confirms the interaction between KLC3 and SLC2A5 proteins in AGS gastric cancer cells. G p38 MAPK Signaling Activation: Western blot analysis reveals the levels of phosphorylated p38 (p-p38) and total p38 proteins in HGC-27, MKN-45, and AGS cells following KLC3 knockdown. ** P < 0.05, ** P < 0.01, *** P < 0.001 indicate statistical significance. H ERK1/2 Signaling Activation: Western blot analysis shows the levels of phosphorylated ERK1/2 (p-ERK1/2) and total ERK1/2 proteins in HGC-27, MKN-45, and AGS cells after KLC3 knockdown. ** P < 0.05, ** P < 0.01, *** P < 0.001 denote statistical significance

    Article Snippet: The PVDF membrane was subsequently blocked to minimize non-specific binding and then incubated with a panel of primary antibodies specific to KLC3, E-cadherin, N-cadherin, vimentin, snail, Wnt1, Wnt3a, CCND1 (all sourced from Abcam, USA), as well as rabbit anti-human GAPDH, Tubulin (also from Abcam, USA), and SLC2A5 (Proteintech Group, Inc.).

    Techniques: Expressing, Quantitative RT-PCR, Biomarker Discovery, Quantitative Proteomics, Gene Expression, Western Blot, Activation Assay, Knockdown

    Reversal of KLC3 Knockdown Effects in AGS Cells by SLC2A5 Overexpression. A Co-immunoprecipitation Analysis of KLC3 and SLC2A5 Interaction: Co-immunoprecipitation experiments were conducted in AGS gastric cancer cells to investigate the physical interaction between KLC3) and SLC2A5. B Restoration of Gene Expression by SLC2A5 Overexpression: Western blot analysis was performed to assess the effects of SLC2A5 overexpression on the downregulation of CCND1, Wnt3a, and Wnt1 caused by KLC3 depletion in AGS cells. C Upregulation of EMT-Related Genes by SLC2A5 Overexpression: qRT-PCR was utilized to examine the expression of EMT-related genes in AGS cells overexpressing SLC2A5. D Restoration of MAPK Pathway Activity by SLC2A5 Overexpression: WB analysis was conducted to investigate the effects of SLC2A5 overexpression on the inhibition of the MAPK pathway caused by KLC3 depletion in AGS cells

    Journal: BMC Cancer

    Article Title: KLC3 drives gastric cancer progression by stabilizing SLC2A5 to activate MAPK signaling and promote epithelial-mesenchymal transition

    doi: 10.1186/s12885-025-15084-x

    Figure Lengend Snippet: Reversal of KLC3 Knockdown Effects in AGS Cells by SLC2A5 Overexpression. A Co-immunoprecipitation Analysis of KLC3 and SLC2A5 Interaction: Co-immunoprecipitation experiments were conducted in AGS gastric cancer cells to investigate the physical interaction between KLC3) and SLC2A5. B Restoration of Gene Expression by SLC2A5 Overexpression: Western blot analysis was performed to assess the effects of SLC2A5 overexpression on the downregulation of CCND1, Wnt3a, and Wnt1 caused by KLC3 depletion in AGS cells. C Upregulation of EMT-Related Genes by SLC2A5 Overexpression: qRT-PCR was utilized to examine the expression of EMT-related genes in AGS cells overexpressing SLC2A5. D Restoration of MAPK Pathway Activity by SLC2A5 Overexpression: WB analysis was conducted to investigate the effects of SLC2A5 overexpression on the inhibition of the MAPK pathway caused by KLC3 depletion in AGS cells

    Article Snippet: The PVDF membrane was subsequently blocked to minimize non-specific binding and then incubated with a panel of primary antibodies specific to KLC3, E-cadherin, N-cadherin, vimentin, snail, Wnt1, Wnt3a, CCND1 (all sourced from Abcam, USA), as well as rabbit anti-human GAPDH, Tubulin (also from Abcam, USA), and SLC2A5 (Proteintech Group, Inc.).

    Techniques: Knockdown, Over Expression, Immunoprecipitation, Gene Expression, Western Blot, Quantitative RT-PCR, Expressing, Activity Assay, Inhibition

    Attenuation of the SLC2A5 gene expression in fibrosarcoma cells. (A) . Images of scratch test (left) and quantitative analysis of closure (right) as a function of time for HT1080tdT and HT1080tdT-ε2A5 fibrosarcoma cells (n = 3). Scale bar 10 µm. (B) . Images of colonies formed by HT1080tdT and HT1080tdT-ε2A5 cells and analysis of colony number (500 cells/well plated at time 0 h). * p = 0.007; n = 3. Colonies were fixed with 70% ethanol and visualized by staining with 1% trypan blue. (C) . Proliferation assay of HT1080tdT and HT1080tdT-ε2A5 cells. HT1080tdT and HT1080tdT-ε2A5 cells were plated at a density of 5.0 × 10 3 cells/well of a 96 well plates. Fructose was added to the media as indicated in the Figure. Cells were cultured for 48 h followed by MTS assay (n = 3). All data in the Figure is representative of more than 3 experiments with 3 biological replicates.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration

    doi: 10.3389/fcell.2022.896297

    Figure Lengend Snippet: Attenuation of the SLC2A5 gene expression in fibrosarcoma cells. (A) . Images of scratch test (left) and quantitative analysis of closure (right) as a function of time for HT1080tdT and HT1080tdT-ε2A5 fibrosarcoma cells (n = 3). Scale bar 10 µm. (B) . Images of colonies formed by HT1080tdT and HT1080tdT-ε2A5 cells and analysis of colony number (500 cells/well plated at time 0 h). * p = 0.007; n = 3. Colonies were fixed with 70% ethanol and visualized by staining with 1% trypan blue. (C) . Proliferation assay of HT1080tdT and HT1080tdT-ε2A5 cells. HT1080tdT and HT1080tdT-ε2A5 cells were plated at a density of 5.0 × 10 3 cells/well of a 96 well plates. Fructose was added to the media as indicated in the Figure. Cells were cultured for 48 h followed by MTS assay (n = 3). All data in the Figure is representative of more than 3 experiments with 3 biological replicates.

    Article Snippet: Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.

    Techniques: Gene Expression, Staining, Proliferation Assay, Cell Culture, MTS Assay

    Editing of the SLC2A5 gene. (A) . Inactivation of the SLC2A5 gene in human MIA-PaCa-2 and HT1080tdT cells using CRISPR/Cas9-mediated in situ gene editing. Guide RNAs were targeted to Exon 3. Genomic DNA of edited cells was sequenced, and positive clones were identified as having a 62 base pair disruption, leading to an early stop codon 14 amino acids downstream of the disruption point. (B) . qPCR analysis of SLC2A5 mRNA abundance in MIA-PaCa-2 and MIA-PaCa-ε2A5 cells (clones B3 and F11) (n = 3). DNA primers were designed to amplify Exon 12 of the SLC2A5 gene. Immunoblot analysis was carried out with antibodies directed at the C-terminus of SLC2A5. Anti-β-tubulin or anti-GAPDH antibodies were used to assess amounts of loaded protein samples. (C) . qPCR analysis of SLC2A5 mRNA abundance in HT1080tdT and HT1080tdT-ε2A5 cells. DNA primers and control antibodies were as described above. (D) . qPCR analyses of SLC2A1, SLC2A2, and SLC2A4 mRNA abundance in MIA-PaCa-2 and MIA-PaCa-ε2A5 cells (n = 3). (E) . qPCR analyses of SLC2A1, SLC2A2, and SLC2A4 mRNA abundance in HT1080tdT and HT1080tdT-ε2A5 cells (n = 3). All data in the Figure is representative of more than 3 biological replicates. NS, not significant.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration

    doi: 10.3389/fcell.2022.896297

    Figure Lengend Snippet: Editing of the SLC2A5 gene. (A) . Inactivation of the SLC2A5 gene in human MIA-PaCa-2 and HT1080tdT cells using CRISPR/Cas9-mediated in situ gene editing. Guide RNAs were targeted to Exon 3. Genomic DNA of edited cells was sequenced, and positive clones were identified as having a 62 base pair disruption, leading to an early stop codon 14 amino acids downstream of the disruption point. (B) . qPCR analysis of SLC2A5 mRNA abundance in MIA-PaCa-2 and MIA-PaCa-ε2A5 cells (clones B3 and F11) (n = 3). DNA primers were designed to amplify Exon 12 of the SLC2A5 gene. Immunoblot analysis was carried out with antibodies directed at the C-terminus of SLC2A5. Anti-β-tubulin or anti-GAPDH antibodies were used to assess amounts of loaded protein samples. (C) . qPCR analysis of SLC2A5 mRNA abundance in HT1080tdT and HT1080tdT-ε2A5 cells. DNA primers and control antibodies were as described above. (D) . qPCR analyses of SLC2A1, SLC2A2, and SLC2A4 mRNA abundance in MIA-PaCa-2 and MIA-PaCa-ε2A5 cells (n = 3). (E) . qPCR analyses of SLC2A1, SLC2A2, and SLC2A4 mRNA abundance in HT1080tdT and HT1080tdT-ε2A5 cells (n = 3). All data in the Figure is representative of more than 3 biological replicates. NS, not significant.

    Article Snippet: Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.

    Techniques: CRISPR, In Situ, Clone Assay, Disruption, Western Blot, Control

    Attenuation of the SLC2A5 gene expression in pancreatic cancer cells. (A) . Images of scratch test (left panel) and quantitative analysis of closure (right panel) for MIA-PaCa-2 and MIA-PaCa-ε2A5 cells (clone B3; ** p = 0.002, n = 3). Scale bar 10 µm. (B) . Scratch test analysis for MIA-PaCa-2 and MIA-PaCa-ε2A5 cell lines clone F11 (** p = 0.001, n = 3). Scale bar 10 µm. (C) . Colony formation images and colony number for MIA-PaCa-ε2A5 cells, clone B3 (left panel; 1000 cells/well plated at time 0 h; * p = 0.0001; n = 3) and clone F11 (right panel; 500 cells/well plated at time 0 h; * p = 0.001; n = 3). (D) . Fructose-dependent proliferation of the MIA-PaCa-2 and MIA-PaCa-ε2A5 cells. MIA-PaCa-2 and MIA-PaCa-ε2A5 (clones B3 and F11) cells were plated at a density of 1.0 × 10 4 cells/well of a 96 well plates. Fructose was added to the culture medium as indicated in the figure. The absorbance at 490 nm (OD 490 ) of the culture medium after MTS assay at time 0 and 48 h in culture are shown. Experiments were performed a minimum of three times in triplicates. * p = 0.0002; n = 3.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration

    doi: 10.3389/fcell.2022.896297

    Figure Lengend Snippet: Attenuation of the SLC2A5 gene expression in pancreatic cancer cells. (A) . Images of scratch test (left panel) and quantitative analysis of closure (right panel) for MIA-PaCa-2 and MIA-PaCa-ε2A5 cells (clone B3; ** p = 0.002, n = 3). Scale bar 10 µm. (B) . Scratch test analysis for MIA-PaCa-2 and MIA-PaCa-ε2A5 cell lines clone F11 (** p = 0.001, n = 3). Scale bar 10 µm. (C) . Colony formation images and colony number for MIA-PaCa-ε2A5 cells, clone B3 (left panel; 1000 cells/well plated at time 0 h; * p = 0.0001; n = 3) and clone F11 (right panel; 500 cells/well plated at time 0 h; * p = 0.001; n = 3). (D) . Fructose-dependent proliferation of the MIA-PaCa-2 and MIA-PaCa-ε2A5 cells. MIA-PaCa-2 and MIA-PaCa-ε2A5 (clones B3 and F11) cells were plated at a density of 1.0 × 10 4 cells/well of a 96 well plates. Fructose was added to the culture medium as indicated in the figure. The absorbance at 490 nm (OD 490 ) of the culture medium after MTS assay at time 0 and 48 h in culture are shown. Experiments were performed a minimum of three times in triplicates. * p = 0.0002; n = 3.

    Article Snippet: Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.

    Techniques: Gene Expression, Clone Assay, MTS Assay

    Restoration of SLC2A5 function via SLC2A5 transgene expression. Proliferation assay (A) and scratch test assay (B) of MIA-PaCa-2, MIA-PaCa-ε2A5 (clone F11) and MIA-PaCa-ε2A5 cells expressing wild-type SLC2A5 or the non-functional SLC2A5-E401A mutant (* p = 0.002; n = 3). In (A) , fold change in cell number is shown at day 0 to day 3. In (B) , the percentage of open scratch is shown at time 0 and 48 h. Proliferation assay (C) and scratch test assay (D) of HT1080tdT, HT1080tdT-ε2A5 cells transfected with expression vectors encoding wild-type SLC2A5 or the non-functional SLC2A5-E401A mutant (* p = 0.001, n = 3). In (C) , the absorbance at 490 nm (OD 490 ) of the culture medium after MTS assay from time 0 and 7 h in culture is shown. In (D) , the percentage of open scratch is shown at time 0 and 7 h. NS, not significant. All data in the Figure is representative of more than 3 experiments with 3 replicates.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration

    doi: 10.3389/fcell.2022.896297

    Figure Lengend Snippet: Restoration of SLC2A5 function via SLC2A5 transgene expression. Proliferation assay (A) and scratch test assay (B) of MIA-PaCa-2, MIA-PaCa-ε2A5 (clone F11) and MIA-PaCa-ε2A5 cells expressing wild-type SLC2A5 or the non-functional SLC2A5-E401A mutant (* p = 0.002; n = 3). In (A) , fold change in cell number is shown at day 0 to day 3. In (B) , the percentage of open scratch is shown at time 0 and 48 h. Proliferation assay (C) and scratch test assay (D) of HT1080tdT, HT1080tdT-ε2A5 cells transfected with expression vectors encoding wild-type SLC2A5 or the non-functional SLC2A5-E401A mutant (* p = 0.001, n = 3). In (C) , the absorbance at 490 nm (OD 490 ) of the culture medium after MTS assay from time 0 and 7 h in culture is shown. In (D) , the percentage of open scratch is shown at time 0 and 7 h. NS, not significant. All data in the Figure is representative of more than 3 experiments with 3 replicates.

    Article Snippet: Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.

    Techniques: Expressing, Proliferation Assay, Wound Healing Assay, Functional Assay, Mutagenesis, Transfection, MTS Assay

    Mitochondria localization and dynamics in SLC2A5-deficient cancer cells. (A) . Electron microscopy analysis of MIA-PaCa-2 cells shows that mitochondria were localized in the center of these cells in the perinuclear space (encircled). In MIA-PaCa-ε2A5 cells, mitochondria were spread throughout the cell towards the cell periphery, and appeared elongated (arrows) (n = 3). (B) . and (C) . Mitochondria area (B) and length (C) analyses in MIA-PaCa-2 and MIA-PaCa-ε2A5 cells. * p < 0.0006; ** p < 0.0001 (n = 3). (D) . Confocal images of mitochondria stained with MitoTracker ® Green in red fluorescent protein-labelled HT1080tdT cells,HT1080tdT-ε2A5 cells or HT1080tdT-ε2A5 cells expressing wild-type SLC2A5. Time-lapse video is shown in the (arrowhead). (E) . Mitochondria number and (F) . mitochondria length in fibrosarcoma HT1080tdT cells, HT1080tdT-ε2A5 or HT1080tdT-ε2A5 cells expressing wild-type SLC2A5. *** p < 0.0002 (n = 3).

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration

    doi: 10.3389/fcell.2022.896297

    Figure Lengend Snippet: Mitochondria localization and dynamics in SLC2A5-deficient cancer cells. (A) . Electron microscopy analysis of MIA-PaCa-2 cells shows that mitochondria were localized in the center of these cells in the perinuclear space (encircled). In MIA-PaCa-ε2A5 cells, mitochondria were spread throughout the cell towards the cell periphery, and appeared elongated (arrows) (n = 3). (B) . and (C) . Mitochondria area (B) and length (C) analyses in MIA-PaCa-2 and MIA-PaCa-ε2A5 cells. * p < 0.0006; ** p < 0.0001 (n = 3). (D) . Confocal images of mitochondria stained with MitoTracker ® Green in red fluorescent protein-labelled HT1080tdT cells,HT1080tdT-ε2A5 cells or HT1080tdT-ε2A5 cells expressing wild-type SLC2A5. Time-lapse video is shown in the (arrowhead). (E) . Mitochondria number and (F) . mitochondria length in fibrosarcoma HT1080tdT cells, HT1080tdT-ε2A5 or HT1080tdT-ε2A5 cells expressing wild-type SLC2A5. *** p < 0.0002 (n = 3).

    Article Snippet: Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.

    Techniques: Electron Microscopy, Staining, Expressing

    SLC2A5 function is necessary for polarized localization of mitochondria in HT1080tdT human fibrosarcoma cells in vitro and in vivo . (A) mitochondria (green) localization in HT1080tdT or HT1080tdT-ε2A5 (vasculature, grey; blue, DAPI) cells that are extravasating out of the chicken CAM vasculature ( in vivo ). Right panels show all three channels: red fluorescent protein (red), mitochondria (green), nuclei (blue) and vasculature (grey). Time-lapse video is shown in the . (B) . Fluorescent channel intensity along the line scans (dashed arrows in (A) (right panel) indicate the direction of the scan). Green line depicts mitochondria localization. The short arrows in (A) and (B) indicate vascular membrane breaches.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Loss of the fructose transporter SLC2A5 inhibits cancer cell migration

    doi: 10.3389/fcell.2022.896297

    Figure Lengend Snippet: SLC2A5 function is necessary for polarized localization of mitochondria in HT1080tdT human fibrosarcoma cells in vitro and in vivo . (A) mitochondria (green) localization in HT1080tdT or HT1080tdT-ε2A5 (vasculature, grey; blue, DAPI) cells that are extravasating out of the chicken CAM vasculature ( in vivo ). Right panels show all three channels: red fluorescent protein (red), mitochondria (green), nuclei (blue) and vasculature (grey). Time-lapse video is shown in the . (B) . Fluorescent channel intensity along the line scans (dashed arrows in (A) (right panel) indicate the direction of the scan). Green line depicts mitochondria localization. The short arrows in (A) and (B) indicate vascular membrane breaches.

    Article Snippet: Anti-SLC2A5 antibodies were purchased from Boster (Cat# PA 2064) and used at 1:500 dilution.

    Techniques: In Vitro, In Vivo, Membrane